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CHEM 476. Gene Expression Laboratory. 3 units

Term Typically Offered: SP

Prerequisite: BIO/CHEM 475; CHEM 313 or CHEM 371, or graduate standing in Biological Sciences.

Heterologous gene expression of a recombinant protein in a microbial system: gene cloning, construction of expression plasmid, DNA sequence analysis, transformation of microbial host, selection and analysis of transformed host cells, expression and purification of recombinant protein. 1 lecture, 2 laboratories. Crosslisted as BIO/CHEM 476.